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Further, a secondary analysis of a trial in women at high risk for or with existing cardiovascular disease reported that high-dose supplemental B-vitamins (1 mg/day of vitamin B 12 , 50 mg/day of vitamin B6, and 2.5 mg/day of folic acid) for 7.3 years had no effect on risk of non-vertebral fractures (122)

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The monolayer was wounded with a 1,000-μl pipette tip (Watson), washed with media and incubated in growth medium without rh EGF and with 0.25 nM hHGF or 100 nM dimeric macrocycles for 50 h